Bio by Priti

Engelmann’s Experiment (1888): Action Spectrum of Photosynthesis

T.W. Engelmann used Cladophora (a filamentous alga), a prism to split light, and aerobic bacteria to study photosynthesis.

Observation: Bacteria clustered in blue-violet and red light regions, showing maximum oxygen release. Very few gathered in the green region.

Conclusion: Blue and red light are most effective for photosynthesis, while green light is least effective. This experiment demonstrated the action spectrum, aligning with the absorption spectrum of chlorophyll a.

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