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1 week ago | [YT] | 103

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Laboratory Talks

🔬✨ Why should the Calibration results be reversed in detail, not just the word Passed?

Many of us see the word Calibration Passed ✅ and check...
But the truth is that this word alone is not enough!

📌 What is Calibration?

- It is a process done by the device to regulate itself and know the relationship between the concentration of the matter we measure and the OD (Optical Density, Absorbance).

- The device draws the Calibration Curve based on the Calibrator values.

- If the curf looks "accepted", the device tells you "Passed".

- But the word Passed means that the curve is formally accepted, not necessarily that the values are accurate or safe.

⚠️ Where's the danger?

Maybe Cal looks solid but the OD values have a slight deviation.

- This deviation causes the QC (quality adjustment) to start switching or drift after the Cal.

- So you find that the QC is not fixed around the Mean, it starts to go up or go down gradually.

- and the result:
Inaccurate numbers, which means that a patient may get treatment incorrectly based on unreliable results.

🧪 Simplified practical examples:

- QC high level supposed to prefer fixed around a certain value. After Calibration, you find it gradually rising up... It’s called the Drift.

- The low level QC seems stable but in reality there is a small bias that grows with time.

- The reasons could be :

- A mistake in preparing the Calibrator (non-storing focus or storing incorrectly).

- Pipetting or OD reading problems.

- Instrument drift or optics problems.

- Human error to enter data or choose Range.

🛠️ What should be done?

1. Check Cal's OD values along with QC trends before you say "done".

2. Connect the Calibration Curve and QC performance... These two need to make sure each other.

3. If you find QC pizog after Cal Passed, do Troubleshooting:

- Is the problem with the Calibrator itself?
- Does the device need Maintenance or Recalibration?
- Is there a human error in preparation or entry?

4. Don't rely on the word "Passed" only... What we care about is Accuracy + Reliability, not just a physical success.

5. Always link cal and QC trends before results depend on the patient.

👩 ⚕️👨 ⚕️ Practical lesson:

Every Calibration is a very sensitive point... Any negligence in the review may result in incorrect patient results.

The subject is not only scientific, it is also a moral and humanitarian responsibility 🙏

💬 Interactive questions for the audience:

- Have you ever seen QC Drift after Cal Passed? 😅
- What's the worst situation that happened to you that you had to call back or fix the device?
- If you find the QC bezog, what is the first step you do?

✨ Educational information for students:

- Calibration Curve:
The curve that defines the relationship between focus and OD.

- QC Trend:
The line that shows the stability of the long term results.

- OD values:
The light values that the device measures as the basis of calibration.

➡️ Simply:
Call tells the device "how to read", QC tells you "does the device read correctly in the long run". The two must retreat together so that the results remain accurate and safe.

3 weeks ago | [YT] | 10

Laboratory Talks

Ramadan Mubarak to everyone.
May Allah almighty bless us with health and happiness.
May Allah almighty keep us on a path that leads to him and prophet Muhammad pbuh.
May Allah almighty make us good human beings who care for others first.
May Allah almighty keep our intentions honest.
Ameen. Sum ameen 🤲

3 weeks ago | [YT] | 16

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