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The Chem Mind
📖 Big news, Chem Family!
I’ve just released my first book: The Lab Coat Chronicles – Volume 1: The Basics 🧪
This book is designed to bridge the gap between chemistry theory and real lab practice. Inside, you’ll find:
✔️ Lab safety essentials
✔️ How to prepare solutions step by step
✔️ Handling glassware like a pro
✔️ QC basics, indicators & more
It’s perfect for students, fresh graduates, and young professionals who want to boost their lab confidence. 🚀
👉 Read it here: www.researchgate.net/publication/395241542_The_Lab…
#TheChemMind #Chemistry #LabLife #BookLaunch
2 months ago (edited) | [YT] | 1
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The Chem Mind
🔬Why is buffer use essential in HPLC or why we use Buffers in HPLC!!!
Buffers are used in HPLC for several reasons:
1. pH Control:
Buffers help maintain a stable pH level in the mobile phase and lock the pH, which is essential for:
-Ionization of analytes: pH affects the degree of ionization, influencing polarity and interaction with the stationary phase.
- Stationary phase stability: Some columns are sensitive to pH changes.
2. Peak Shape and Resolution:
Consistent pH of buffers can improve peak shape and resolution by:
- Reducing peak tailing
- Increasing column efficiency
- Enhancing separation of analytes
3. Reproducibility:
Buffers ensure consistent pH conditions, which is crucial for:
- Method development and validation
- Repeatable retention times and peak areas
- Reliable quantitation
4. Column Protection:
Buffers can help protect the column from:
- pH-related degradation
- Ionization-induced damage
Common buffers used in HPLC include:
- Phosphate buffers
- Acetate buffers
- Formate buffers
- Ammonium buffers
#HPLC
#chemistry
#lab
2 months ago | [YT] | 1
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The Chem Mind
💡 Did you know?
The element Gallium can melt in your hand because it liquefies at just 29.7°C.
Imagine holding a metal that turns into liquid!
2 months ago | [YT] | 2
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The Chem Mind
Did you know Hydrogen is so light it can escape Earth's gravity?
2 months ago | [YT] | 2
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