Prime editing (pegRNA + RT + nCas9)

Base editing with CRISPR-Cas9

dCas9: CRISPRi CRISPRa CRISPR-ON CRISPR-OFF (Beyond Cas9 Genome Editing)

CRISPR KO vs CRISPR KI experiment using CRISPR-Cas9

How to design and express CRISPR guide RNA [pX330 plasmid, Tutorial]

Genome Editing with CRISPR-Cas9

CRISPR-Cas9-mediated Adaptive Immunity Mechanism in Bacteria [3 Steps of CRISPR-Cas system]

A Brief History of Genome Editing

Protein Expression Vectors - tet-OFF and tet-ON (tTA/rtTA + Tet based gene regulation) [Part 5]

Protein Expression Vectors (pET vector) - Induction of Protein Expression (IPTG + T7 Pol) [Part 4]

Protein Expression Vectors - Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]

Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]

Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]

RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)

Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning

Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning

TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional

Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning

Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer

Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors

P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality

F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)

Lambda Phage vector, Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus

Plasmid and Phagemid cloning vector - Selectable vs. Screening marker

Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes

Restriction Enzymes (Endonucleases) in Molecular Cloning

Genetic Engineering - Classic vs Modern methods

RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps

Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)

Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)

Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)

Telomere DNA Replication - Chromosome end replication problem in eukaryotes

DNA Replication in Space and Time

Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation

DNA Replication (Eukaryote) TERMINATION - Non-specific vs Site-specific Termination Mechanism

DNA Replication (Eukaryote) ELONGATION - Polymerase Switching + different types of DNA polymerase

DNA Replication (Eukaryote) INITIATION - Origin Firing - CMG Helicase forms the Replisome

DNA Replication (Eukaryote) INITIATION - Origin Licensing - cdt1 recruits Helicase Double Hexamer

DNA Replication (Eukaryote) INITIATION - Origin Recognition by ORCs and cdc6

DNA Replication (Prokaryote) TERMINATION - Ter sites and Tus protein together stop DNA replication

DNA Replication ELONGATION - DNA Synthesis Biochemistry

DNA Replication (Prokaryote) ELONGATION - Trombone Model of Elongation + Okazaki Fragment Synthesis

DNA Replication (Prokaryote) INITIATION - Sliding clamp and DNA polymerase loading during INITIATION

DNA Replication (Prokaryote) INITIATION - Helicase and Primase make the RNA Primer during INITIATION

DNA Replication (Prokaryote) INITIATION - Origin Melting by DnaA starts DNA Replication INITIATION

DNA Replication - Origin of Replication (OriC, ORBs, ARS etc.)

DNA Replication - Introduction - Is DNA polymerase slow?

Arabinose (Ara) Operon: araC and DNA looping (deep-dive video)

Tryptophan (Trp) Operon: a repressible operon - UGG codons in E. coli & TRAP protein in B. subtilis

Lactose (Lac) Operon: an inducible operon (deep-dive video)

RNA polymerase I Transcription ELONGATION & TERMINATION - The beauty of rRNA gene termination region

RNA polymerase I Transcription INITIATION - UBF and SL1 function in rRNA transcription

Cap-Independent Translation Initiation - IRES (virus and eukaryote) and uORF

Post-Translational Modifications in prokaryotes and eukaryotes - Types, Functions, and Examples

Eukaryotic Translation TERMINATION - Translation Termination Complex and Ribosome release factors

Eukaryotic Translation ELONGATION - How do eEF1A and eEF2 work? Role of diphthamide in translation?

Eukaryotic Translation INITIATION - 80S ribosome assembly steps | Initiation factors in mRNA loading

Prokaryotic Translation TERMINATION - How do release factors work? Function of RFs, RF3, and RRF

Prokaryotic Translation ELONGATION - How do EF-Tu and EF-G move the ribosome? - Deep-dive Video

Prokaryotic Translation INITIATION - How do 70S ribosomes initiate translation? - Deep-dive Video